Variable Number Tandem Repeats for Global Strain Typing and Strain Differentiation of Mycobacterium leprae
نویسندگان
چکیده
منابع مشابه
Variable numbers of TTC repeats in Mycobacterium leprae DNA from leprosy patients and use in strain differentiation.
Strain differentiation of Mycobacterium leprae would be of great value for epidemiological investigation to identify the infectious sources of leprosy, to understand transmission patterns, and to distinguish between relapse and reinfection. From the M. leprae genome sequence database, TTC DNA repeats were identified. Primer sets designed to amplify the region flanking TTC repeats revealed PCR p...
متن کاملDiversity of potential short tandem repeats in Mycobacterium leprae and application for molecular typing.
A recent advance in molecular typing for tracing the transmission of leprosy is the discovery of short tandem repeats (STRs) in Mycobacterium leprae. To substantiate polymorphic loci from STR as promising candidates for molecular typing tools in leprosy epidemiology, 44 STR loci including 33 microsatellites and 11 minisatellites were investigated among 27 laboratory strains by sequencing PCR pr...
متن کاملMultilocus variable-number tandem repeat typing of Mycobacterium ulcerans.
The apparent genetic homogeneity of Mycobacterium ulcerans contributes to the poorly understood epidemiology of M. ulcerans infection. Here, we report the identification of variable number tandem repeat (VNTR) sequences as novel polymorphic elements in the genome of this species. A total of 19 potential VNTR loci identified in the closely related M. marinum genome sequence were screened in a co...
متن کاملMultiple-locus variable-number tandem repeat analysis for strain typing of Clostridium perfringens.
Clostridium perfringens is ubiquitous in the environment and causes diseases in man and animals, with syndromes ranging from enteritis, enterotoxemia, and sudden death to food poisoning and gas gangrene. Understanding the epidemiology of these infections and of the evolution of virulence in C. perfringens necessitate an efficient, time and cost effective strain typing method. Multiple-locus var...
متن کاملCharacterisation of short tandem repeats for genotyping Mycobacterium leprae.
OBJECTIVE Establish a typing system for Mycobacterium leprae based on polymorphic DNA structures known as short tandem repeats (STR). DESIGN Assess 16 polymorphic STR for sensitivity, specificity and reproducibility in standard assays using reference strains of M. leprae. RESULTS Primers for 16 STR loci were selected based on PCR product size and for their ability to sequence each STR locus...
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ژورنال
عنوان ژورنال: Journal of Medical Microbiology & Diagnosis
سال: 2014
ISSN: 2161-0703
DOI: 10.4172/2161-0703.1000156